The processing of green coffee for the extraction of polyphenols resulted in green coffee spent (GCS) accounting 90% dietary
polysaccharides. The GCS rich in Non-digestible carbohydrates (oligosaccharides) represent promising
prebiotic nutrients. These Non-digestible oligosaccharides were extracted and characterized for valorization of the GCS. The enzymatic (Viscozyme), thermal (roasting) and aqueous (hot water infusion) extraction yielded 19.92?±?2.1% of Mono-oligosaccharides with mannose (6.90?±?0.9?μg/Mg) and
galactose (8.35?±?1.0?μg/Mg) as major components. Further, the GCS oligosaccharides were evaluated for prebiotic efficiency using
Lactobacillus species (
L. plantarum, L. casei and
L. fermentum). The GCS oligosaccharides also stimulated the growth of
Lactobacillus casei (MTCC 1423) and
Lactobacillus fermentum (MTCC 903) by increasing their number from 8.96 Log CFU/ml to 9.82 Log CFU/ml and from 8.97 Log CFU/ml to 9.66 Log CFU/ml respectively. The maximum utilisation of Mannose (70%) and galactose (66%) was observed in
L. plantarum. Additionally GCS oligosaccharides tested for acid resistance and found resistant towards artificial human gastric juice. The non-digestibility of GCS oligosaccharides noted 90% at pH 1. This study provides insights about bio-sustainable approach of use of GCS in designing synbiotic functional food.