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Role of Ascorbic Acid on Mercuric Chloride-Induced Genotoxicity in Human Blood Cultures
Area/Stream: Medicine, Authors: M.V. Rao, N. J. Chinoy, M. B. Suthar, M. I. Rajvanshi Keywords: Mercuric chloride, Genotoxicity, Ascorbic acid, Human blood culturesJournal Name: Toxicology In Vitro, Year:2001, Month:December, Volume: 15, Issue: 6 Page No: 649-654, ISSN: 0887-2333, DOI/Link: https://doi.org/10.1016/S0887-2333(01)00081-9Abstract:
Efforts are made to find therapeutic agents capable of minimizing genotoxicity of various natural and man-made compounds. The genotoxicity induced by mercury compounds remains controversial. Therefore we have investigated the genotoxic effect of mercuric chloride (MC; HgCl2) at three concentrations (1.052, 5.262 and 10.524 ?m) and role of l-ascorbic acid (vitamin C) at a concentration of 9.734 ?m on MC-treated short-term human leucocyte cultures. We assessed the proliferative rate index (PRI), sister chromatid exchange (SCE) and chromosomal aberrations (CAS) in control and MC-treated cultures with and without vitamin C supplementation. The results showed that MC has no effect on cell-cycle kinetics, but the frequency of SCE/cell was significantly higher in a dose-dependent manner than control values. HgCl2 also significantly induced C-anaphases (abnormal mitosis) in blood cultures. These effects were prevented by the addition of vitamin C to MC-treated cultures. The data indicate the mutagenic activity of MC and the protective role of vitamin C on mercury-induced genotoxicity in human blood cultures is probably due to its strong antioxidant and nucleophilic nature.
Cite this: M.V. Rao, N. J. Chinoy, M. B. Suthar, M. I. Rajvanshi,"Role of Ascorbic Acid on Mercuric Chloride-Induced Genotoxicity in Human Blood Cultures", Toxicology In Vitro,, 15, 6, December, 2001, 649-654, 0887-2333, https://doi.org/10.1016/S0887-2333(01)00081-9
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