editedbook
Publication: IIP Proceedings Year: 2022, Month: November Page No: 230-240, ISSN/ISBN: 978-93-95632-87-4, DOI/Link: https://rsquarel.org/assets/docupload/rsl2023452752F44C781A4.pdf
IMPLEMENTATION OF GREEN SOLVENT SYSTEM FOR THE ANALYSIS OF ADAPALENE IN BULK AND TOPICAL GEL FORMULATION BY RP-HPLC
Area/Stream: Pharmacy & Nursing, Authors: Ramreddy Godela, Yagnambhatla Rajendra, Kranthi Kumar Pola, Dr. Beda Durgaprasad, Rani Dumpala Keywords: Adapalene, HPLC, Validation, Stability indicating method, Sensitivity. Isocratic elution. Book Name /series: Futuristic Trends in Pharmacy & Nursing, Volume 2, Book 25, Part 2, Chapter 3Publication: IIP Proceedings Year: 2022, Month: November Page No: 230-240, ISSN/ISBN: 978-93-95632-87-4, DOI/Link: https://rsquarel.org/assets/docupload/rsl2023452752F44C781A4.pdf
Abstract:
A simple and rapid stabilityindicating, RP-HPLC method was ascertained to determine adapalene in bulk and gel formulation. Separation was achieved on enable C18 column (150 x 4.6mm; 5μm) under the isocratic mode of elution by using mobile system mixture of green solvents tetrahydrofuran and methanol (30:70 V/V). The flow rate was maintained at 1.0 ml/min with runtime 10min. UV detection was done at 360 nm. The method was found to be linear for series concentration ranges from 20-100µg/ml. The limit of detection and quantification were found to be 3.27and 0.025µg/ml. Results of precision and accuracy obtained were within the limits. The suggested approach was effectively employed for the determination of adapalene in a labelled formulation (gel), with a % assay of 99%. The suggested method's stability-indicating capacity is shown by evaluating forced degradation samples in which the peak purity of adapalene is determined as well as the resolution of degradant from the analyte peak. The collected findings demonstrate that the provided approach is a stability indicating method. The stated HPLC method has been verified in terms of specificity, precision, sensitivity, accuracy, linearity, and robustness according to ICH.
Cite this: Ramreddy Godela, Yagnambhatla Rajendra, Kranthi Kumar Pola, Dr. Beda Durgaprasad, Rani Dumpala,"IMPLEMENTATION OF GREEN SOLVENT SYSTEM FOR THE ANALYSIS OF ADAPALENE IN BULK AND TOPICAL GEL FORMULATION BY RP-HPLC", Futuristic Trends in Pharmacy & Nursing, Volume 2, Book 25, Part 2, Chapter 3, November, 2022, 230-240, 978-93-95632-87-4, https://rsquarel.org/assets/docupload/rsl2023452752F44C781A4.pdf
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